Isolate 48886
EMH-24-23-54 University of St. ThomasESKAPE Test
| Tested against ESKAPE relative | Antibiotic activity | Length of incubation | Media used |
|---|---|---|---|
| Bacillus subtilis | Yes | 24.0 hr | Brain Heart Infusion (BHI) |
| Escherichia coli | Yes | 24.0 hr | Brain Heart Infusion (BHI) |
| Pseudomonas putida | No | ||
| Mycobacterium smegmatis | Not tested | ||
| Enterbacter aerogenes | Not tested | ||
| Acinetobacter baylyi | Not tested | ||
| Staphylococcus epidermidis | Not tested | ||
| Enterococcus raffinosus | Not tested |
Distinguishing Characteristics
On the E.coli plate, only one isolate exhibit antibiotic activity, with a very small zone of inhibition. On the B.subtilis plate, four isolates exhibit antibiotic activity, with very small zones of inhibition.
16S rRNA Test
Genus
PseudomonasFASTA sequence
TCCAGTGGTAATTGGAAATGGGGCGAAGCCTGATCCAGCCATGCCGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACTTTAAGTTGGGAGGAAGGGTACTTACCTAATACGTGAGTATTTTGACGTTACCGACAGAATAAGCACCGGCTAACTCTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTGGTTCGTTAAGTTGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCCAAAACTGGCGAGCTAGAGTAGGGCAGAGGGTGGTGGAATTTCCTGTGTAGCGGTGAAATGCGTAGATATAGGAAGGAACACCAGTGGCGAAGGCGACCACCTGGGCTCATACTGACACTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGTAAGTTCCCACCGCAAAATTCAAATTAAGGTCAAAAAGAACCCTTTTTTTTCCCCCCCCCCCCCGCGGTTCCTTTCTATTTCTTCCATAAAAAAGAGGAGGGGAGATTCCCCCCCTCCAATCTCACCCCTTTAGGATATCCATCCGAATGTGACCCGCADistinguishing Characteristics
99% identity between query and subject sequence. 16Sr and 16Sf primers were used during PCR.
Soil Sample 47603
Collected Thursday February 9, 2023
Culture Conditions 47837
Recorded Thursday February 16, 2023
Isolate 48886
Recorded Thursday March 16, 2023
Culture Conditions 47847
Recorded Thursday February 16, 2023
Isolate 48888
Recorded Thursday March 16, 2023
Culture Conditions 47841
Recorded Thursday February 16, 2023
Isolate 48897
Recorded Thursday March 16, 2023
Isolate 48889
Recorded Thursday March 16, 2023
Culture Conditions 48883
Recorded Thursday March 16, 2023
Isolate 48898
Recorded Thursday March 16, 2023
Isolate 48896
Recorded Thursday March 16, 2023
Isolate 48894
Recorded Thursday March 16, 2023
Isolate 48893
Recorded Thursday March 16, 2023
Isolate 48892
Recorded Thursday March 16, 2023