Soil Sample 225 / Culture 252 / Isolate 308
Isolate 308
S19BIO145TBR41+ California State University-Sacramento
Published
ESKAPE Test
Published
Recorded Wednesday, March 6, 2019
Tested against ESKAPE relative Antibiotic activity Length of incubation Media used
Bacillus subtilis No 48.0 hr R2A
Erwinia carotovora Yes 48.0 hr R2A
Agrobacterium tumefaciens Yes 48.0 hr R2A
Mycobacterium smegmatis Not tested
Enterbacter aerogenes Not tested
Pseudomonas putida Not tested
Acinetobacter baylyi Not tested
Escherichia coli Not tested
Staphylococcus epidermidis Yes 48.0 hr R2A
Enterococcus raffinosus Yes 48.0 hr R2A
Distinguishing Characteristics
Antibiotic production seen in all ESKAPE organisms tested, except for B. subtilis
16S rRNA Test
Published
Recorded Monday, April 8, 2019
Genus
Bacillus
FASTA sequence
302B_051_F10_519R_C08.seq DOWNLOAD FILE
Distinguishing Characteristics
96.01%
Antibiotic Activity of Extract
Published
Recorded Tuesday, April 30, 2019
Solvent used
Ethyl Aaetate
Experiment Description
Top Agar Method. Dried extract was resuspended in 200 uL of methanol. 20 uL spotted onto each plate and dried on 10%TSBA. 7mL Top agar media (LB broth) inoculated with 100 uL of safe ESKAPE organisms and poured onto plate.
Tested against ESKAPE relative Antibiotic activity Length of incubation Media used
Agrobacterium tumefaciens Yes 48.0 hr LB
Erwinia carotovora No
Bacillus subtilis No
Enterococcus raffinosus No
Staphylococcus epidermidis Yes 48.0 hr LB
Escherichia coli No
Acinetobacter baylyi Yes 48.0 hr LB
Pseudomonas putida No
Enterbacter aerogenes No
Mycobacterium smegmatis Not tested
Eukaryotic Inhibition
Published
Recorded Monday, May 6, 2019
Experiment description
20uL of solvent (S19BIO145TBR41+ extract, methanol, cyclohexamide) on each quadrant containing 3 seeds on water agar in triplicates. Grown for 2 weeks near sunny window.
Eukaryotic organism Eukaryotic inhibition
Radish seed No
Associated Entries

Soil Sample 225
Collected Tuesday March 5, 2019

Published

Culture Conditions 252
Recorded Wednesday March 6, 2019

Published

Isolate 305
Recorded Wednesday March 6, 2019

Published

Isolate 284
Recorded Wednesday March 6, 2019

Published

Isolate 280
Recorded Wednesday March 6, 2019

Published

Isolate 311
Recorded Wednesday March 6, 2019

Published

Isolate 310
Recorded Wednesday March 6, 2019

Published

Isolate 304
Recorded Wednesday March 6, 2019

Published

Isolate 303
Recorded Wednesday March 6, 2019

Published

Isolate 302
Recorded Wednesday March 6, 2019

Published

Isolate 308
Recorded Wednesday March 6, 2019

Published

Isolate 307
Recorded Wednesday March 6, 2019

Published

Isolate 306
Recorded Wednesday March 6, 2019

Published