Soil Sample 68832 / Culture 68846 / Isolate 68855
Isolate 68855
Pine 3 Devil's Lake Beloit College
Published
ESKAPE Test
Published
Recorded Tuesday, June 25, 2024
Tested against ESKAPE relative Antibiotic activity Length of incubation Media used
Erwinia carotovora Yes 48.0 hr nutrient agar
Bacillus subtilis Yes 48.0 hr nutrient agar
Enterococcus raffinosus No
Staphylococcus epidermidis No
Escherichia coli No
Acinetobacter baylyi No
Pseudomonas putida No
Enterbacter aerogenes No
Mycobacterium smegmatis Yes 48.0 hr nutrient agar
16S rRNA Test
Published
Recorded Tuesday, June 25, 2024
Genus
Prestia
FASTA sequence
GGGGCAGGGCTTAGCTAATACATGCAAGTCGAGCGAACTGATTAGAAGCTTGCTTCTATGACGTTAGCGGCGGACGGGTTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACTTCGGGAAACCGAAGCTAATACCGGATAGGATCTTCTCCTTCATGGGAGATGATTGAAAGATGGTTTCGGCTATCACTTACAGATGGGCCCGCGGTGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCATAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGCTTTCGGGTCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACGAGAGTAACTGCTCGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGAAAAGCGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGGCTTTTTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACTCTAGAGATAGAGCGTTCCCCTTCGGGGGACAAAGTGACAGGTGGTGCATGGTTGTCNTCAGCTCGGGCCTGAGATGTTGGGTTAGTCCCGCAACGAGGCACCCTTGATCTATTGCCACATTTAGTGGGCNTCTAAGGGACTGCCGGGACAACCGGAGAAGGGGGGATAAGCAATTTCTGCCCTTTGACTGGGTCCACCGGTAAA
Distinguishing Characteristics
Percent Identity: 98.27% Universal 16S rRNA bacterial primers 27F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1392R (5’-GGTTACCTTGTTACGACTT-3’)]
Antibiotic Activity of Extract
Published
Recorded Tuesday, June 25, 2024
Solvent used
ethyl acetate
Experiment Description
The experiment followed the Chemical Extraction protocol by Kristin Labby and Katherine Maloney. Extracts were reconstituted in methanol and tester bacteria were spread on LBA plates. The reconstituted extract was spotted on the spread plate in 10uL amounts. Methanol was spotted in the same amount as the extract for a negative control. A TE 30 (tetracycline) disk was used as a positive control. Plates were incubated at 30C for 24 hours.
Tested against ESKAPE relative Antibiotic activity Length of incubation Media used
Erwinia carotovora Yes 192.0 hr LBA
Bacillus subtilis Yes 192.0 hr LBA
Enterococcus raffinosus Not tested
Staphylococcus epidermidis Not tested
Escherichia coli Not tested
Acinetobacter baylyi Not tested
Pseudomonas putida Not tested
Enterbacter aerogenes Not tested
Mycobacterium smegmatis Not tested
Associated Entries

Soil Sample 68832
Collected Tuesday June 25, 2024

Published

Culture Conditions 68846
Recorded Tuesday June 25, 2024

Published

Isolate 68855
Recorded Tuesday June 25, 2024

Published