Soil Sample 68821 / Culture 68822 / Isolate 68825
Isolate 68825
Avon 6 burr oak Beloit College
Published
ESKAPE Test
Published
Recorded Tuesday, June 25, 2024
Tested against ESKAPE relative Antibiotic activity Length of incubation Media used
Erwinia carotovora No
Bacillus subtilis Yes 48.0 hr nutrient agar
Enterococcus raffinosus No
Staphylococcus epidermidis Yes 48.0 hr 20% TSA
Escherichia coli Yes 48.0 hr nutrient agar
Acinetobacter baylyi No
Pseudomonas putida No
Enterbacter aerogenes No
Mycobacterium smegmatis No
16S rRNA Test
Published
Recorded Tuesday, June 25, 2024
Genus
Bacillus
FASTA sequence
TGCTATACATGCAAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTAAGTCCCGCAACGAGCGCACCCTTGATCTAGTTGCCGCATTCAGTGGGCCTCTAAGTGACTGCCGTGACAACCGGAGGAAGGGGGGATGACGTCAATCTTCTGCCCTATGACTGGGTACCCCGGCTAA
Distinguishing Characteristics
Percent Identity: 98.56% Universal 16S rRNA bacterial primers 27F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1392R (5’-GGTTACCTTGTTACGACTT-3’)]
Antibiotic Activity of Extract
Published
Recorded Tuesday, June 25, 2024
Solvent used
ethyl acetate
Experiment Description
The experiment followed the Chemical Extraction protocol by Kristin Labby and Katherine Maloney. Extracts were reconstituted in methanol and tester bacteria were spread on LBA plates. The reconstituted extract was spotted on the spread plate in 10uL amounts. Methanol was spotted in the same amount as the extract for a negative control. A TE 30 (tetracycline) disk was used as a positive control. Plates were incubated at 30C for 24 hours.
Tested against ESKAPE relative Antibiotic activity Length of incubation Media used
Bacillus subtilis Yes 192.0 hr nutrient agar
Staphylococcus epidermidis Yes 192.0 hr nutrient agar
Escherichia coli Yes 192.0 hr nutrient agar
Mycobacterium smegmatis Not tested
Enterbacter aerogenes Not tested
Pseudomonas putida Not tested
Acinetobacter baylyi Not tested
Enterococcus raffinosus Not tested
Associated Entries

Soil Sample 68821
Collected Tuesday June 25, 2024

Published

Culture Conditions 68822
Recorded Tuesday June 25, 2024

Published

Isolate 68825
Recorded Tuesday June 25, 2024

Published