Soil Sample 89 / Culture 288 / Isolate 289
Isolate 289
California State University-Sacramento
Published
ESKAPE Test
Published
Recorded Wednesday, March 6, 2019
| Tested against ESKAPE relative | Antibiotic activity | Length of incubation | Media used |
|---|---|---|---|
| Bacillus subtilis | Yes | 48.0 hr | 10% Tryptic Soy Agar (TSA) |
| Enterococcus raffinosus | Not tested | ||
| Staphylococcus epidermidis | Yes | 48.0 hr | 10% Tryptic Soy Agar (TSA) |
| Escherichia coli | Not tested | ||
| Acinetobacter baylyi | No | ||
| Pseudomonas putida | Not tested | ||
| Enterbacter aerogenes | No | ||
| Mycobacterium smegmatis | Not tested |
Distinguishing Characteristics
Isolate 21 is observed to have antibiotic production against Gram positive organisms (S. epidermidis, B. subtilus) and another soil isolate as well. Isolate 2 produces antibiotics against B. subtilus. Isolate 11 has antibiotic production against B. subtilus. All antibiotic producers are against Gram positive organisms.
16S rRNA Test
Published
Recorded Wednesday, March 27, 2019
Genus
BacillusFASTA sequence
GACCGACAGGTGCCGCTTATTCACTAGCACTTGTTCTTCCCTAACAACAGAGTTTTACGACCCGAAAGCCTTCATCACTCACGCGGTGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGGGTCTCAGTCCCAGCGTGGCCGATCACCCTCTCAGGTCGGCTACTCATCGTTGCCTTGGTGAGCCGTTACCTCACCAACTAGCTAATGCGACGCGGGTCCTTCCATAAGTGACAGCCGAAGCCGCCTTTCAATTTCGAACCATGCAGTTCATAATATTATCCGGCATAAGACCCGGTTTCCCGGAGTTATCCCAGTCTTATGGGCAGGTTACCCACGTGTTACTCACCCGTGCGCCGCTAACTTCTTGAGAGCAAGATCTCAATCCATTCGCTCGACTTGCATGTATTAGGCACGCCGCGTGCGTTCATCCTGAGCC
E10_RDP_Output.txt
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Distinguishing Characteristics
Bacillus sp. strain AzoEpi19 % ID = 96.62%16S rRNA Test
Published
Recorded Wednesday, March 27, 2019
Genus
Bartonella sp.FASTA sequence
CTCGGGTACCGTCATGATCTTCGCCGATGAAACAGCTATTTTTCTTTCCGGATTCATCGCTCTAGCGCCCTGACTGCTTCTTGATTGCGCCATATGCCAGTATTCCCCTCTGCTGCCTCGCGCAAT
E9_RDP_Output.txt
DOWNLOAD FILE
Distinguishing Characteristics
% ID = 74.95 % 27F, 1492R16S rRNA Test
Published
Recorded Wednesday, March 27, 2019
Genus
Propionibacterium acnesFASTA sequence
TTACCGTCACTCACGCGTCGTACACAGCTTGAAAGCGGTTTACAACCCGTAGGCCGTCTTCCCGCACGCGGCGTTGTTGCATCAGGATTCCGCACATTGTGCAATATTCCCGACTGCTACCTCCCGTAGAAGTCTGGTGGTATCTCAGTCCAATGTGGCCAATCACCCTCTCAGGCCGGCTACCCGTCAAAGCCTTGGTCGCCACTACCCCACCTTTCAGCTGATAAGCCGCGAGTCCATCCCCAACCGCCGAAACTTTCCAACCCACACCATGGCCCACCCCGGGATTA
E8_RDP_Output.txt
DOWNLOAD FILE
Distinguishing Characteristics
Percent identity: 90.61% Primers: 27F, 1492RAntibiotic Activity of Extract
Published
Recorded Tuesday, April 9, 2019
Solvent used
Ethyl AaetateExperiment Description
Secondary metabolite extracted with ethyl acetate and tested via top agar assay. No positive results observed. Perhaps issue with solvent extraction or null-production of antibiotic from the initial lawn assay.| Tested against ESKAPE relative | Antibiotic activity | Length of incubation | Media used |
|---|---|---|---|
| Bacillus subtilis | No | ||
| Enterococcus raffinosus | No | ||
| Staphylococcus epidermidis | No | ||
| Escherichia coli | No | ||
| Acinetobacter baylyi | No | ||
| Pseudomonas putida | No | ||
| Enterbacter aerogenes | No | ||
| Mycobacterium smegmatis | No |
Associated Entries
Soil Sample 89
Collected Thursday February 14, 2019
Published
Culture Conditions 288
Recorded Wednesday March 6, 2019
Published
Isolate 289
Recorded Wednesday March 6, 2019
Published