Soil Sample 22820 / Culture 26253 / Isolate 26254
Isolate 26254
SP21 BIO45 ER30 California State University-Sacramento
Published
ESKAPE Test
Published
Recorded Monday, April 26, 2021
| Tested against ESKAPE relative | Antibiotic activity | Length of incubation | Media used |
|---|---|---|---|
| Bacillus subtilis | No | ||
| Enterococcus raffinosus | Not tested | ||
| Staphylococcus epidermidis | Yes | 120.0 hr | 10% Tryptic Soy Agar (TSA) |
| Escherichia coli | No | ||
| Acinetobacter baylyi | No | ||
| Mycobacterium smegmatis | Not tested | ||
| Enterbacter aerogenes | Not tested | ||
| Pseudomonas putida | Not tested |
Distinguishing Characteristics
Antibiotic producer #30 is a mixed sample. Poor lawn growth occurred, but antibiotic activity of isolate #30 can still be observed. In the above picture, please note the small zone of clearing that formed around isolate #30 on the S. epidermidis plate. Additionally, the colony morphology of Isolate #30 can be observed. Upon streaking producer #30, two different types of colonies were observed - one that was more beige in color and the other a pale yellow. One, likely the more beige colony, appears to produce an extrapolysaccharide. BIOCHEMICAL TESTS: Gram stain = G- (but can clearly see it is a mixed sample by the larger, more purple [thus indicating G+ for those bacteria] cells). 3% KOH = watery test (negative test result) indicated G+ (the opposite of the Gram stain; likely due to mixed culture). Blood Agar = gamma hemolysis (no hemolysis). TSI Agar = red slant (K) / red butt (K) (neither glucose, sucrose, not lactose fermented; no hydrogen gas; no hydrogen sulfide). Starch Agar = no exoenzymes. Catalase = positive. Oxidase = negative.
Associated Entries
Soil Sample 22820
Collected Monday February 15, 2021
Published
Culture Conditions 26253
Recorded Monday April 26, 2021
Published
Isolate 26254
Recorded Monday April 26, 2021
Published